Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU
نویسندگان
چکیده
منابع مشابه
HSV1-tk gene expression imaging agents
In this study, we synthesized Tc(CO)3-2 0-aminomethylpyridyl-20-deoxyuridine (Tc(CO)3-AMPDU) and Tc(CO)3-aminoethylpyridyl-20-deoxyuridine (Tc(CO)3-AEPDU) as potential agents for imaging the expression of the non-invasive herpes simplex virus type-1 thymidine kinase. AMPDU and AEPDU were synthesized from uridine in five chemical steps and then labeled with [Tc(CO)3(H2O)3] + (370MBq/0.5mL) at 10...
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To optimize the sensitivity of imaging HSV1-tk/GFP reporter gene expression, a series of HSV1-tk/GFP mutants was developed with altered nuclear localization and better cellular enzymatic activity, compared to that of the native HSV1-tk/GFP fusion protein (HSV1-tk/GFP). Several modifications of HSV1-tk/GFP reporter gene were performed, including targeted inactivating mutations in the nuclear loc...
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متن کاملComparison of radiolabeled nucleoside probes (FIAU, FHBG, and FHPG) for PET imaging of HSV1-tk gene expression.
UNLABELLED The efficacy of 3 radiolabeled probes of current interest for imaging herpes simplex virus type 1 thymidine kinase (HSV1-tk) expression in vivo with PET, including (124)I- or (131)I-labeled 2'-fluoro-2'-deoxy-1-beta-D-arabinofuranosyl-5-iodouracil (FIAU), (18)F-labeled 9-[4-fluoro-3-(hydroxymethyl)butyl]guanine (FHBG), and (18)F-labeled 9-[3-fluoro-1-hydroxy-2-propoxymethyl]guanine (...
متن کاملTitration of variant HSV1-tk gene expression to determine the sensitivity of 18F-FHBG PET imaging in a prostate tumor.
UNLABELLED Because of its high selectivity and specificity for the imaging reporter probe 9-(4-(18)F-fluoro-3-[hydroxymethyl]butyl)guanine ((18)F-FHBG), the herpes simplex virus type 1 thymidine kinase (HSV1-tk) variant sr39tk is actively being studied as a PET reporter gene. We recently demonstrated the capability of using a prostate-specific transcriptional amplification PET reporter vector, ...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2007
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2007.49